Effectiveness of Red Onion Extract (Allium Cepa) on the Elongation of Papillae and Neutrophil Cell in the Gastroesophageal Junction (GEJ) of Sprague Dawley Strain Rats Gastroesophageal Reflux Disease (GERD) Model
), Vetinly .(2), Tena Djuartina(3), Riki Tenggara(4), Iskandar R. Budianto(5),
(1) Faculty of Medicine and Health Sciences of Atma Jaya University, Jakarta, Indonesia
(2) Department of public health and nutrition sciences of Atma Jaya University, Jakarta, Indonesia
(3) Anatomy department of Atma Jaya University, Jakarta, Indonesia
(4) Internal medicine department of Atma Jaya University, Jakarta, Indonesia
(5) Surgery department of Atma Jaya university, Jakarta, Indonesia
Corresponding Author
Abstract
Background: Gastroesophageal acid reflux disease (GERD) is a disorder caused by the reflux of stomach acid when the lower esophageal sphincter (LES) relaxes with a prevalence of around 1.03 billion worldwide. One of the supporting examinations carried out to diagnose GERD is a histopathological examination. This study aims to determine the effect of red onion extract on the papillae elongation and neutrophil cells of the gastroesophageal junction in Sprague Dawley rats.
Methods: This experimental study was conducted on Sprague Dawley rats induced with 1 mL of HCl + 1 mL of ascorbic acid, administered into the gastric cardia of the rats, mixed with 1 mL of sodium thiocyanate (NaSCN) and 1.5 mL of sodium nitrite (NaNO₃) to develop a GERD model. The rats were then divided into ten groups with different treatments, including red onion extract (250, 500, 750 mg/kgBW) and PPI (pantoprazole).
Results: It was stated that there was a significant relationship between the effect of red onions and changes in papillae elongation in rats induced by GERD (P-value <0.05). The neutrophil cells showed no significance on the impact of red onion (p-value >0,05). Nor are the papillae elongation and the neutrophil cell changes dose-dependent.
Conclusion: A significant reduction in the histopathological appearance of papillae elongation was seen in the majority of the population. However, no significant results were found for the different doses of shallot extract.
Keywords: Gastroesophageal acid reflux disease, allium cepa, papillae elongation, red onion extract, neutrophil cells
Keywords
References
Clarrett DM, Hachem C. Gastroesophageal Reflux Disease (GERD). Mo Med. 2018;115(3):214-218.
Kataria J, Rivera D, Grin A, Reed DE, Rodrigues D. The role of histology in the diagnosis of non‐erosive reflux disease: A systematic review and meta‐ analysis. Neurogastroenterology and Motility.2023.
Nirwan JS, Hasan SS, Babar ZU, Conway BR, Ghori MU. Global Prevalence and Risk Factors of Gastro-oesophageal Reflux Disease (GORD): Systematic Review with Meta-analysis. Sci Rep. 2020;10(1):5814. Published 2020 Apr 2. doi:10.1038/s41598-020-62795-1
Varid, Varid KA. Gambaran Karakteristik penderita penyakit gerd (gastroesophageal reflux disease) Yang Berobat di Puskesmas Kawatuna Kelurahan Kawatuna, Kota Palu [Internet]. PERPUSTAKAAN FAKULTAS KEDOKTERAN. Studi D-III Keperawatan; 2020
Nehlig A. Effects of Coffee on the Gastro-Intestinal Tract: A Narrative Review and Literature Update. Nutrients. 2022;14(2):399. Published 2022 Jan 17. doi:10.3390/nu14020399.
Hidalgo, A., Chilvers, E. R., Summers, C., & Koenderman, L. (2019). The Neutrophil Life Cycle. Trends in immunology, 40(7), 584–597. https://doi.org/10.1016/j.it.2019.04.013
Haastrup PF, Thompson W, Søndergaard J, Jarbøl DE. Side Effects of Long- Term Proton Pump Inhibitor Use: A Review. Basic Clin Pharmacol Toxicol. 2018;123(2):114-121. doi:10.1111/bcpt.13023
Lennicke C, Cochemé HM. Redox metabolism: ROS as specific molecular regulators of cell signaling and function. Mol Cell. 2021;81(18):3691-3707. doi:10.1016/j.molcel.2021.08.018.
Turshudzhyan A, Samuel S, Tawfik A, Tadros M. Rebuilding trust in proton pump inhibitor therapy. World J Gastroenterol. 2022;28(24):2667-2679. doi:10.3748/wjg.v28.i24.2667Brigelius-Flohé R, Maiorino M. Glutathione peroxidases. Biochim Biophys Acta. 2013;1830(5):3289-3303. doi:10.1016/j.bbagen.2012.11.020.
Windari T. Peranan Ekstrak Bawang Dayak ( Eleutherine palmifolia ) Sebagai Agen Anti Tukak Lampung (Peptic Ulcer) Pada Tikus Wistar (Rattus norvegicus) Jantan yang Diinduksi Etanol. Jurnal Pangan dan Agroindustri.2017;5(1):61-70.
Marefati N, Ghorani V, Shakeri F, et al. A review of anti-inflammatory, antioxidant, and immunomodulatory effects of Allium cepa and its main constituents. Pharm Biol. 2021;59(1):287-302. doi:10.1080/13880209.2021.1874028
Xu D, Hu MJ, Wang YQ, Cui YL. Antioxidant Activities of Quercetin and Its Complexes for Medicinal Application. Molecules. 2019;24(6):1123. Published 2019 Mar 21. doi:10.3390/molecules24061123.
Indonesian Society of Gastroenterology. National consensus on the management of gastroesophageal reflux disease in Indonesia. Acta Med Indones. 2014;46(3):263-271.
Matrella ML, Valletti A, Marra F, Mallamaci C, Cocco T, Muscolo A. Phytochemicals from Red Onion, grown with eco-sustainable fertilizers, protect mammalian cells from oxidative stress, increasing their viability. Molecules. 2022;27(19):6365.
Maret-Ouda, J., Markar, S. R., & Lagergren, J. (2020). Gastroesophageal Reflux Disease: A Review. JAMA, 324(24), 2536–2547.
Arifin WN, Zahiruddin WM. Sample Size Calculation in Animal Studies Using Resource Equation Approach. Malays J Med Sci. 2017 Oct;24(5):101-105. doi: 10.21315/mjms2017.24.5.11. Epub 2017 Oct 26. PMID: 29386977; PMCID: PMC5772820.
Takubo, K., Honma, N., Aryal, G., Sawabe, M., Arai, T., Tanaka, Y., Mafune, K., & Iwakiri, K. (2005). Is there a set of histologic changes that are invariably reflux associated?. Archives of pathology & laboratory medicine, 129(2), 159–163.
Dunbar KB, Agoston AT, Odze RD, et al. Association of Acute Gastroesophageal Reflux Disease With Esophageal Histologic Changes. JAMA. 2016;315(19):2104–2112. doi:10.1001/jama.2016.5657
Sies H. Role of metabolic H2O2 generation: redox signaling and oxidative stress. J Biol Chem. 2014;289(13):8735-8741. doi:10.1074/jbc.R113.544635
Liew PX, Kubes P. The Neutrophil's Role During Health and Disease. Physiol Rev. 2019;99(2):1223-1248. doi:10.1152/physrev.00012.2018
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